Ann Res Antioxid. 2019;4: e02.
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  PDF Download: 32

Original Article

Comparison of protein-binding malondialdehyde level between breast cancer, fibroadenoma and normal tissues in cases of breast surgery; a pilot study

Soheila Sayad 1, Zahra Mahmoudi 2,3, Seyyed Amir Yasin Ahmadi 2, Reza Nekouian 4* ORCID logo

1 Department of Breast Surgery, Firoozgar Clinical Research Development Center, Iran University of Medical Sciences, Tehran, Iran
2 Student Research Committee, Iran University of Medical Sciences, Tehran, Iran
3 Department of Medical Biotechnology, Iran University of Medical Sciences, Tehran, Iran
4 Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
*Corresponding Author: Reza Nekouian, Email: nekouian.r@iums.ac.ir

Abstract

Introduction: Breast cancer is the most common malignant solid tumor in women. Like other solid tumors, an inflammatory microenvironment with oxidative stress provides a suitable condition for tumor growth.

Objectives: This pilot study was designed to compare the protein-binding level of malondialdehyde (MDA) – as an oxidative stress index – among breast cancer, fibroadenoma and normal tissues in Iranian cases of breast surgery.

Materials and Methods: A total of 17 surgical cases were selected in operation room including 6 cases of invasive ductal carcinoma (breast cancer), 6 cases of fibroadenoma and 5 cases of mammoplasty surgery (control group). All the cancer patients had received neoadjuvant chemotherapy. The total protein was extracted from tissues and then the level of MDA was studied using spectrophotometry.

Results: Concentration of MDA was 46.84 (±11.90), 53.78 (±1.69) and 52.96 (±2.29) µM in invasive ductal carcinoma, fibroadenoma and control groups, respectively. According to analysis of variance (ANOVA) the differences were not statistically significant (P=0.238).

Conclusion: MDA level was not different between the groups of study. This lack of association might be due to the wide distribution of data in cancer group which in turn might be resulted from personalized responses of the tumors to neoadjuvant chemotherapy.


Citation: Sayad S, Mahmoudi Z Ahmadi SAY, Nekouian R. Comparison of protein-binding malondialdehyde level between breast cancer, fibroadenoma and normal tissues in cases of breast surgery; a pilot study. Ann Res Antioxid. 2019;4:e02.

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Submitted: 07 Aug 2019
Accepted: 29 Nov 2019
ePublished: 15 Dec 2019
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