Abstract
Introduction: Renal ischemia reperfusion (I/R) occurs during renal surgical procedure that leads to renal injuries and can progress to acute renal failure.
Objectives: Due to central role of reactive oxygen species (ROS) in pathogenesis of renal I/R, rosmarinic acid (RA) an antioxidant agent were used against renal I/R injuries.
Materials and Methods: Forty male mature Sprague Dawley rats (180–200 g) were divided into five groups (8 rats per group) as follows; group 1 control; group 2 I/R, without treatment; group 3 I/R + RA (50 mg/kg); group 4 I/R + RA (100 mg/kg) and group 5 I/R + RA (200 mg/kg). RA was given orally by gavage in groups 3, 4 and 5 one hours before surgery. I/R induced through ligation of renal pedicles bilaterally for 45 minutes and then 24-hour reperfusion. After 24 hours, blood were sampled, Left kidney fixed in formal saline solution and then tissue section (5 µ thickness) prepared and were stained with PAS method. Serum malondialdehyde (MDA), urea and creatinine were measured. Histological variables were assessed morphometrically and semiquantitativly. The data compared by SPSS 13 and Mann-Whitney U test at P<0.05.
Results: Histological injuries (tubular necrosis, casts, diameter, tubular volume density, mean glomerular volume) and serum creatinine, urea, MDA were improved in RA treated groups in comparison with group 1 (P<0.05), but the treatment cannot be kept variables at the same level as that of control group.
Conclusion: Histopathological injuries of renal I/R, were reduced by RA via inhibition of lipid peroxidation and finally led to amelioration of serum urea and creatinine.